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The second reaction cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to create ferric enzyme, NO, citrulline, and water as products (Fig. 9) - UV/Vis/NIR. As drawn in Fig - https://www.slideshare.net/julieanndesalorenz30. 9, the reaction might likewise include electron transfer from H4B to the Fe, IIO2 intermediate


l9 As displayed in Fig. 9, this could be due to the fact that the H4B extreme serve as an electron sink in a later point in the reaction, so regarding permit NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.